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  • Environmental DNA metabarcoding has recently emerged as a non destructive alternative to traditional sampling for characterising species assemblages. We here provide a consistent dataset synthetizing all eDNA sampling sites in French Guiana to date. We also provide a taxon by site matrix for fishes presence / absence as inferred from eDNA. Our aim is to allow a transparent communication to the stakeholders, and provide the foundation for a monitoring program based on eDNA.